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The recombinant strain was first grown in 5 ml MRS medium at initial pH of 4.0, 5.0 and 6.0.
The cells suspension was added in the test medium at initial OD600 of 0.05, and the flasks were then shaken at 180 rpm.
Cultured cells were inoculated in 5 mL of the same medium at initial OD660 = 0.2 and incubated until OD660 reached 1.0.
Cellulase production induced by wheat bran medium and cellulose was performed in a 500 mL flask with 100 mL fluid medium at initial pH 5.5, 30°C, and 200 rpm, using 20 h mycelia pre-grown on 1× Vogel's medium with glucose (2%, w/v) as a sole carbon source.
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The strain was cultivated in 250 mL conical flasks containing 25 mL medium inoculated at initial OD of 0.016 and maintained for 24 h at 37°C and 200 rpm.
An experiment was performed with food waste medium at an initial concentration of 129 g/L and fermentation was initiated.
Yeast cells were inoculated in 20 mL of YPGN50 or 100 medium at an initial OD600 of 0.1.
Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 °C for 24 h in the shake flasks.
The resuspended yeast cells were used to inoculate 5 l shake flask with 1 l fresh NLM medium at an initial OD600 nm of 0.1, and cultivated at 30 °C and 200 rpm for 120 h.
The precultures were used to inoculate 100 ml shake flask with 20 ml fresh minimal medium at an initial OD600 nm of 0.1 that were then cultivated for 72 h at 30 °C and 200 rpm.
The single and binary effects of initial Remazol Turquoise Blue-G (RTBG) reactive dye and initial copper II) concentrations on the dye or/and copper II) bioaccumulation efficiency of C. tropicalis was investigated in 10 g l−1 molasses sucrose containing growth medium at an initial pH value of 4.0 and optimized using response surface methodology (RS M.
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