Exact(5)
Twenty-four hours after seeding the cells, SFN was added to the culture medium at indicated concentrations and the maximum concentration of DMSO was 0.1% (v/v) in the medium.
Myostatin was added to conditioned medium at indicated final concentration prior to myoblasts incubation.
All NSAID and cisplatin were dissolved in dimethyl sulphoxide and added to the medium at indicated concentrations.
Original Western blots to Figure 6 Expression of Phosphorylated CREB in Astrocytes and Neurons after stimulation with the active compound hMNCapo sec or control medium: Cultured human Astrocytes and Neurons were incubated with hMNCapo sec or control (cell culture-) medium at indicated concentrations.
After stimulation with 500 nM ADDLs in NeuroBasal medium at indicated time points, the cultures were collected in cold homogenization buffer (in mM: 50 MOPS (pH 7.4), 320 sucrose, 100 KCl, 0.5 MgCl2, 0.2 DTT, 50 NaF, 20 NaPPi, 20 β-glycerophosphate, 1 EDTA, 1 EGTA, 1 PNPP-Na, 1 Na3VO4, 0.5 PMSF,10 μg/ml leupeptin, 10 μg/ml aprotin, 10 μg/ml pepstatin A, 100 μg/ml benzamidine).
Similar(55)
At Day 3, CSC-cbR was changed to fresh CSC-cbR, CSC-HC, or CSC-free medium, as indicated at the center of the illustration.
Same amount of ethanol without SM was added to control neuronal cultures; (ii) incubation of ASMko neurons with Bacillus aureus Smase (Sigma-Aldrich) at 0.1 unit/100 μl medium (as indicated in Galvan et al, 2008).
Cellular toxicity was evaluated by measuring lactate dehydrogenase (LDH) activity in the medium at each indicated time point after exposure to the CytoTox96 nonradioactive assay (Promega, Madison, WI, USA).
The release profile of the platinum (II) complexes in phosphate buffered saline and RPMI-1640 liquid medium at 37 °C indicated sustained manner of drug release with no initial burst effect.
Methylmethanesulfonate (MMS) (Sigma-Aldrich) was added to the culture medium at the indicated concentrations.
24 h after transfection doxorubicin or 5-fluorouracil (5FU) were added in the medium at the indicated doses.
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