Sentence examples for medium at designated from inspiring English sources

For metabolic labelling, [S]methionine was added to the culture medium at designated times for a period of 6 h.

Samples of the release medium were removed at designated times using a sample probe with an inline 0.45-μm filter to prevent removal of the microparticles during sampling.

Self-organized time division multiple access (STDMA) is a deterministic MAC protocol, whereby vehicles can access wireless medium at their designated time slots.

For addition to tissue culture, DA-6 was dissolved in water and added to the medium at the designated concentrations before autoclaving.

At designated times, treatment medium was suctioned off and cells were lysed with 50 µL lysis buffer (10 mM HEPES; 2 mM EDTA; 0.1% CHAPS; pH 7.4) to which 1 mM DTT (dithiothreitol; 1 2,000, freshly prepared; Sigma-Aldrich) was added.

At designated time intervals, the medium was removed, and the wells were washed twice with PBS.

At designated time intervals, the medium was removed, and the wells were washed with PBS for two times.

At designated time intervals, the medium was removed and 20 μl/well of 5 mg/ml MTT solution was added to each well.

At designated time points, culture medium was removed from the wells, and the samples were washed three times with warm PBS.

At designated time intervals, the PBS medium was replaced, and an ultraviolet-visual spectrophotometer (Cary 100) was used to measure the former medium's absorbance around 241 nm.

For B.subtilis cultures growing exponentially in DSM medium at 37°C, 100 μl of cells were taken at designated times.