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The sterile MNPs were dissolved in DMEM medium at concentrations of 20, 100, and 500 μg/mL.
The nanoparticles were well-dispersed in the culture medium at concentrations of 100, 250, and 500 μg/ml.
Reactivation yields were increased with respect to the control (fully aqueous medium) when cosolvents were added to the reactivation medium at concentrations below 50% (v/v).
Then, Lf-M-PAEEP-PLLA-NPs or M-PAEEP-PLLA-NPs was added in cell culture medium at concentrations of 5, 10, 15, and 20 μg/mL.
Similarly, MWCNT dispersed in a growth medium at concentrations of 10, 20 or 40 mg/L enhanced seed germination and biomass production [100].
The study of Carmen et al.[10] reported that suspensions of TiO2 nanoparticles prepared in U937 cells culture medium at concentrations that covered a range (0.005 to 4 mg/kg) induced apoptosis in 24 and 48 h.
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The nanoparticles were dispersed in the medium at concentration of 100, 250 and 500 (g/ml).
To visualize chromosomes, Hoechst33342 (Sigma) was added to the medium at concentration of 5 10 ng/ml.
Plant extracts were added to the top layer medium at concentration of 100 μg/mL.
5-Aza-dC (Sigma, St . Louis MO, USA) was added to the fresh medium at concentration of 1, 5, and 10 μM in duplicate and cells were harvested after 5 days.
For the assays, the extract was concentrated to dryness in a lyophilizer, the dried preparation was homogenized in culture medium at concentration of 10 mg/ml (stock solution), and filtered through a 0.45 μM Millipore.
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