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An experiment was performed with food waste medium at an initial concentration of 129 g/L and fermentation was initiated.
In general, total internal reflection takes place at the boundary between two transparent media when a ray of light in a medium of higher index of refraction approaches the other medium at an angle of incidence greater than the critical angle.
Yeast cells were inoculated in 20 mL of YPGN50 or 100 medium at an initial OD600 of 0.1.
In the growth curve, drawn in laccase medium at an interval of 6 h up to 48 h of incubation, the maximum biomass was attained at 24 h that afterwards showed decline up to 48 h.
The resuspended yeast cells were used to inoculate 5 l shake flask with 1 l fresh NLM medium at an initial OD600 nm of 0.1, and cultivated at 30 °C and 200 rpm for 120 h.
The precultures were used to inoculate 100 ml shake flask with 20 ml fresh minimal medium at an initial OD600 nm of 0.1 that were then cultivated for 72 h at 30 °C and 200 rpm.
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Evaporative cooling is able to generate the cooling medium at a temperature approaching to the ambient wet bulb temperature.
The cells were transferred to YPD medium at a starting OD600 of 0.1 and incubated at 30°C with shaking.
Briefly, freshly isolated human monocytes or cryopreserved monocytes were resuspended in medium at a concentration of 0.5 × 106 cells/ml.
The carbon source was added into medium at a concentration of 2%% (w/v or v/v).
The nanoparticles were dispersed in the medium at a concentration of 100, 250, and 500 μg/mL.
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