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Briefly, freshly isolated human monocytes or cryopreserved monocytes were resuspended in medium at a concentration of 0.5 × 106 cells/ml.
The carbon source was added into medium at a concentration of 2%% (w/v or v/v).
The cells were transferred to YPD medium at a starting OD600 of 0.1 and incubated at 30°C with shaking.
The nanoparticles were dispersed in the medium at a concentration of 100, 250, and 500 μg/mL.
The propagation of seismic waves in the fractured medium at a certain time disturbs the enhanced permeability of the fractures.
Cycloheximide was added directly to the culture medium at a final concentration of 10 µM.
Nuclear counterstaining was done by incorporating DAPI (Invitrogen) into the 80% glycerol mounting medium at a concentration of 14.3 µM.
Cells were washed and resuspended in NSC sorting medium at a concentration of 5 million cells/ml.
The PPARγ antagonist, GW9662 (Alexis Biochemicals), was added to the adipogenic differentiation medium at a concentration of 0.5 µM.
To select for plasmid pCD1Ap, ampicillin was added into the medium at a concentration of 25 µg/ml.
Recombinant protein Wif1 was added to the culture medium at a final concentration of 3nM at the indicated time intervals.
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CEO of Professional Science Editing for Scientists @ prosciediting.com