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Non-supplemented DW medium should not be confused with DW basic medium (as used in [11-14,16]) whicontainsins 10 g/L (NH4 2SO4.
This medium consisted of the same basal medium as used for the CD34 expansion step supplemented with 10% HS, the low-dose cytokine cocktail (as previously mentioned) and a new high-dose cytokine cocktail consisting of 20 ng/ml IL-7, 22 ng/ml SCF, 1000 U/ml IL-2 (Proleukin®; Chiron, München, Germany) and 20 ng/ml IL-15 (CellGenix).
The constructs were cultured for 7 days in medium as used for expansion (control medium).
The anolyte (hexacyanoferrate(II)) and catholyte (phosphate-buffered medium, as used in Rozendal et al. 2008) recycle speed was 60 mL/min.
Substrate utilisation by the isolated strains was determined in duplicate in the same bicarbonate-buffered medium as used for enumeration and isolation but without yeast extract.
It is important to note that in all cases the effect of oxalate was evaluated using the same culture medium as used for the corresponding control.
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A 0% FBS medium was used as RF-M1, whereas a 40% FBS medium was used as RF-M2 [ 14, 122].
YES or YEA medium was used as complete medium, and EMM2 medium, containing nutritional supplements when necessary, was used as minimal medium.
Dulbecco's modified Eagle's medium (DMEM) was used as basal medium.
DMEM culture medium was used as negative control.
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