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BeWo and JEG-3 cells transfected with pre-miR-34a or pre-Scramble were seeded at a density of 20 cells/cm in normal culture medium as stated as the above and allowed to grow for 2 weeks.
MDA-MB-231 cells were transiently transfected with AM9D or control DNAzyme in serum-free medium as stated above.
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The amount of particles detected on top of the cells appeared to be positively correlated with NP concentration and was much greater in the presence of serum-free medium than in the presence of serum supplemented medium although as stated above, in absence of serum no agglomeration of NPs was detected by light scattering and analytical centrifugation analyses.
Cells were maintained overnight at 37 °C in 5% CO2 for 18 h in growth medium supplemented as stated in the figure legends.
The effects of permeability and porosity of the porous medium as well as state of charge were investigated on the standard deviation of the temperature field and maximum temperature inside the battery in all four cases.
Cells were seeded in 96-well plates in medium with supplements as stated above, 5,000 cells/well, 100 μl/well and incubated overnight.
Approximately 50% confluent cells were given new medium, with supplements as stated, 24 h before lysing in RIPA (radioimmunoprecipitation assay) buffer.
As stated earlier, medium-colored drapes with a white plastic backing can reduce solar heat gain by as much as 33percentt if left closed during hot months.
In the synthesis of CuO nanowires, when copper acetate reacted with sodium hydroxide in the aqueous medium, the following reaction takes place as stated in Equation 1.
The culture medium was replaced and cells treated as stated in the figure legends.
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