Sentence examples for medium as required from inspiring English sources

Under these conditions, cells attached to the bottom of the plates, where they were left to differentiate for 20 additional days, changing the medium as required.

Just before adding to cultured cells, curcumin was diluted in culture medium as required keeping the final DMSO concentration at 1%. Whole blood assay was performed as previously described [57].

Further dilution was done in cell culture medium as required.

The appropriate amino acid stocks were added to minimal medium as required.

Cells were subcultured by rinsing gently in PBSA and then in 250 μg/ml trypsin and 200 μg/ml EDTA in PBSA; they were incubated until completely detached, and resuspended and diluted in growth medium as required.

Yeast transformants were grown overnight in an SC-Ura or SC-Ura-Leu medium, as required, at 24°C and then the culture was refreshed in YEPD to 0.2 OD600 and grown at 24°C for 6 h.

Bacteria were grown on Iso-sensitest agar/broth (Oxoid, Basingstoke, UK), Tryptic Soya agar/broth (TSA/TSB; Oxoid, Basingstoke, UK) Luria Bertani agar/broth (LBA/LB; Sigma-Aldrich, St . Louis MO, USA) or minimal medium (BSM [ 51]) as required.

The medium was replaced every 2-3 dass, as required.

Growth was monitored daily by hemocytometer, and the cell density was adjusted as required with fresh medium containing CpdA and Tet.

After overnight incubation, the MEF medium was replaced with hESC medium, and thereafter, the medium was changed either every day or every other day, as required.

Medium was changed every 2 3 days, and sub-culturing was as required.