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Isolation of this strain was performed using KG minimal medium as previously described (Chong et al. 2016).
The Aminobacter sp. strain MSH1 was cultivated in MSNCopt medium, as previously described in Schultz-Jensen et al. (2014).
Bone marrow and spleen cells were recovered and suspended in culture medium, as previously described [5].
Spores were prepared by growth on Duncan and Strong agar medium as previously described [17], [18].
Human oral carcinoma cells (KB) were cultured in RPMI 1640 medium as previously described.
Juvenile cultures were derived from mononucleate ascospores incubated on germination medium as previously described [19].
Membranes were then washed and bacteria transferred to selective medium as previously described [22].
After isolation, islets were cultured in M-199 culture medium as previously described [52].
The membranes were then washed and the bacteria transferred to selective medium as previously described [12].
The cells were cultured in complete medium as previously described [17], [51].
Yeast forms were maintained by cultivation at 37°C in Ham's F-12 medium as previously described [29].
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