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Corrosion studies were carried out using salt spray apparatus for different time period (50,100,150, 200hrs) in 5% NaCl fog medium as per ASTM B117 standard.
Corrosion studies were carried out in boiling nitric acid medium as per ASTM A262 practice-C test and three phase corrosion test and the results were compared with Cp-Ti and Ti 5Ta 1.8Nb alloy.
If sounds were either sound waves in the medium surrounding the object, or items dependent on the medium as per the Relational Event Theory, we would be forced to admit that the tuning-fork started and ceased to sound; because the relevant sound waves would not be present in the surrounding medium.
Thus, the force acting on the atoms of the medium as per Hook's law is given as begin{array}{l}Falpha -x F=-Kxend{array} (2 where K is a constant depending on the nature of the medium on which the wave propagates and the intermolecular interaction.
Cells were transfected with 100 pmoles of siRNA per well in a six-well plate using Lipofectamine 2000 Reagent (Invitrogen, Carlsbad, CA) in antibiotic free growth medium, as per the manufacturer's instructions.
The following day, cells were co-transfected with NF-κB luciferase reporter gene construct (pNF-κB-LUC and p-Renilla Stratagene, Santaclara, CA) (in a 10∶1 ratio) using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA) in serum-free IMDM medium as per manufacturer protocol.
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H. hispanica was cultivated at 37 °C in nutrient-rich medium AS-168 (per liter: 5.0 g Bacto Casamino Acids, 5.0 g yeast extract, 1.0 g sodium glutamate, 3.0 g trisodium citrate, 200 g NaCl, 20 g MgSO4 · 7H2O, 2.0 g KCl, traces of FeSO4 · 4H2O and MnCl2 · 4H2O, pH 7.2), and 3 μg/ml of mevinolin was added when required [ 44].
Large airports are defined as having seen more than 20 million seats per year, medium as having between 10 and 20 million and small as being under 10 million.
Bioreactor culture yielded ∼40-fold more EV per mL conditioned medium, as compared to conventional cell culture.
The vaccination strain used was M. bovis BCG Danish strain 1331 (SSI, Copenhagen, Denmark) which was reconstituted from freeze-dried stocks stored at 4°C in Sauton's medium supplied as per instructions.
Sterilized kernels were dried, arranged in a 5 × 5-grid on modified NB medium (MNB) as per Chen et al. [ 85] and incubated in the dark at 27°C.
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