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Ampicillin (50 μg/ml) or kanamycin (50 μg/ml) was added to the medium as needed.
Ampicillin (50 μg/ml) was added to the medium as needed.
Pure glycerol or crude glycerol were added to the defined medium as needed.
Ampicillin (50 μg ml−1) was added to the medium as needed.
For low-dose inocula, the viral stock was further diluted with Bartels Medium as needed.
RA was stored as a solution (10 mM in DMSO) and diluted in growth medium as needed.
Similar(48)
Partially cover and bring to a boil over medium-high heat, then reduce the heat to medium-low or as needed so the water is barely bubbling at the edges.
Medium was replaced every 4 5 days or as needed.
Beat on medium speed for about 2 minutes, until smooth, stopping to scrape down the bowl, as needed.
Mix the dough on medium-low speed for 10 minutes, stopping to scrape down the dough from the sides of the bowl and the hook, as needed.
Cook for about 5 minutes, reducing the heat to medium-low as needed to keep the mixture from scorching.
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