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Exact(7)
Shared medium: As mentioned in Section 3, in wireless ad hoc networks, the medium is shared and, as a result, TCP faces problems such as channel contention and unfairness.
Seeds of the transgenic lines and wild type were sterilized and sown on germination medium as mentioned above.
PANC-1 human pancreatic carcinoma and HT29 human colon adenocarcinoma grade II cell lines were cultured in medium as mentioned here for PDAC cell lines.
The human Saos-2 osteosarcoma and the HeLa cervical carcinoma cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in the same medium as mentioned above.
The absence of anti-HA labeling of BV-G2/p17 virions in immuno-EM was consistent with the absence of detectable scFvG2/p17 protein in the extracellular medium, as mentioned above.
Since both strains, Δire-1 and Δhac-1, are unable to utilize cellulose, both mutants and their parental strains were pre-grown in minimal medium (1× Vogel's salts, 2% w/ v sucrose) for 16 h, and then young hyphae were harvested and transferred into cellulose medium as mentioned above for another 4 h to induce cellulase gene expression.
Similar(53)
The "Minimal medium" composition as mentioned in section 'Fermentation medium and cultivation' was used, and yeast cells of 16 g/l were added in the fermenter in the case of free cells cultivation.
Bundle up as mentioned.
For each strain, an overnight culture (C-medium, 37°C) was diluted 1∶20 in fresh C medium (modified as mentioned above) and 1 ml was added to each well of a 24-well plate.
The production medium was prepared as mentioned above where bacterial isolate no.
The medium used was as mentioned previously with glucose (1 % w/v) equivalent to CMC as the only carbon source.
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