Exact(1)
The BEAS-2B immortalised human bronchial epithelial cell line (Shanghai Institutes of Biological Sciences, Shanghai, China) was cultured in LHC-9 medium as instructed by the provider.
Similar(59)
I did as instructed.
Ruklick did as instructed.
Gould called as instructed.
Do as instructed.
Then do as instructed.
Furthermore, AC2M2 cells were transfected with 10 nM non-silencing control or ezrin small interfering RNA (siRNA) using Lipofectamine 2000 reagent and Opti-MEM-I reduced serum medium (Invitrogen) as instructed by the manufacturer.
Briefly, cocktails of different amounts of antisense oligonucleotide and transfection reagents (ifect, MoleculA) were made in the presence of 300 μl of the culture medium (without supplements) by incubating for 30 min, as instructed by the manufacturer.
The rayon swabs were inserted into the Virocult medium immediately after sampling and the tip of the tube was squeezed as instructed by the manufacturer.
Resume cooking as instructed.
Fans dressed, as instructed, in all black.
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