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For growth inhibition experiments on agar plates the same mineral salt medium as for liquid cultures was used with supplementation with 2% agar to obtain similar conditions.
Suspension cultures were prepared by adding approximately 1 g of callus to 30 ml of liquid culture medium (as for callus induction but excluding agar), in 100 ml Erlenmeyer flasks enclosed with cotton wool bungs, and shaking at 110 rpm on a reciprocating shaker.
Cultures for ATP measurements were grown in the same medium as for the respiration experiment in a light∶dark (12∶12 hrs) cycle.
HTCC1062 cells used for morphological analysis were grown in the same medium as for the respiration experiment, in either continuous light (70 µmol photons m−2 s−1) or continuous darkness.
UMCL01-101 and BCBL-1 were maintained in the same medium as for 2F7 but without sodium pyruvate.
The cells were cultured with the same medium as for microvascular ECs, but VEGF-C was added to the medium.
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Clearly, they think there's a bright future for the medium as well.
The constructs were cultured for 7 days in medium as used for expansion (control medium).
In this case, stations keep sensing the medium (listening) for this additional time, after detecting the medium as idle for a DIFS interval.
Palm oil was added to a waste glycerol based medium as precursor for the lipopeptides.
Spironucleus vortens was grown axenically in TYI-S33 medium as modified for Giardia at 27°C [ 38].
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