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For transcript analyses, strains were grown in minimal medium as described (Nowrousian et al. 2005).
Amyloid fibril formation was confirmed in R10 medium as described in the Supplemental methods.
After 16 h serum starvation, cells were incubated with treatment medium (as described above).
After collagen polymerization, 1.0 ml of treatment medium (as described above) was added atop each collagen gel lattice.
After 16 h serum starvation, cells were incubated with treatment medium (as described above) for 48 h before immunostaining.
Later medium was changed into the basic RPE medium as described above.
The cultures were enriched with f/2 medium as described by Guillard (1975).
The strain was grown on Standard I Medium as described previously (Corvini et al. 2004c).
Re-sterilised capsules were placed in vials containing IVC medium as described above.
R. opacus was grown in minimal medium as described in the material and methods section.
MSH1 was grown in MSNCopt medium and PM2 in OPM medium, as described above.
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