Exact(5)
The bacterial growth was measured at 620 nm using uninoculated medium as blank.
Cell growth was determined by optical density at 600 nm in an Agilent HP 8453 spectrophotometer using the fermentation medium as blank.
100 µl aliquots of supernatants were used to determine LDH release with phenol red free medium as blank control.
With MEM medium as blank and untreated HeLa cells as control, we measured each concentration of these compounds in triplicate.
Two types of controls, only culture medium as blank and wells with unlabelled cells as background were also set.
Similar(55)
Before cell proliferation assay, wells of all groups were washed with PBS for two times, replaced with fresh culture medium, and set one new group with culture medium only as blank control.
For bioreactor inocula, E. coli strains BL21(HO1) and BL21 mHO1) were grown in 50mL of LB medium plus 100μg mL-1 ampicillin or kanamycin, respectively, in 250mL capacity Erlenmeyer flasks with rotary shaking (225 rpm) at 37o C to an A600 of 2 to 6 with LB medium as a blank control.
Flasks containing no cells were also incubated with substrate and serum-free medium as assay blanks.
Medium containing 500 µg/ml FITC-inulin was used as a reference of the initial experimental conditions, and medium without FITC-inulin as blank.
Fluorescence measurements were taken at 488/522 excitation/emission and TAP medium was used as blank.
Complete medium was used as blank.
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