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In addition, the concentrations of estradiol and progesterone in culture medium are measured.
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PGE2 in the culture medium was measured by ELISA.
The amounts of lactate and the pH value (acid) of the PLGA and PLLA degradation medium were measured.
The amount of endostatin released by the cells into the medium was measured using an ELISA.
The activity of MMP secreted into culture medium was measured by zymography using gelatin and collagen as substrates, as described previously52.
After 16 h incubation, glucose and lactate concentrations in the culture medium were measured using the Glucose Assay Kit and l-Lactate Assay Kit (Biovison, Milpitas, CA, USA), respectively.
Nitrite and nitrate levels in the culture medium were measured, and inducible-type NO synthase (iNOS) and nitrotyrosine were determined by immunofluorescence staining.
The attenuation of 59.5 keV gamma-rays from the Am-241 through the hydrogen medium was measured by using an HPGe detector.
h Adiponectin level in medium were measured by ELISA.
Cytokine concentrations in culture medium were measured and expressed as mean ± SD of three independent experiments.
The content of CyA in the medium was measured by HPLC method as described above.
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