Hydrolysis experiments carried out using thin glass tanks filled with glass beads to construct a hypothetical and inert, homogeneous porous medium were analysed using a 2D numerical model.
The corrosion inhibition properties of a new class of oxadiazole derivatives, namely 3,5-bis n-pyridyl -1,2,4-oxadiazoles (n-DPOX) for C3,5-bis n-pyridyl -1,2,4-oxadiazoles medium were analysed by electrochemical impedance spectroscopy (EIS).
The influences of the current density, pH, additives, and concentration medium were analysed.
Insulin concentration in the medium was analysed using a radioimmunoassay kit specific for rat/mouse insulin (Linco, St Charles, MO, USA).
Cytokine levels in the cell culture medium were analysed with a SECTOR Imager 2400 reader (MesoScale Discovery, Gaithersburg, Maryland, USA).
viciae 3841and the prsD (A895), toaD (A913), tobD (A896), virB6 (autA), A1010(autB0), A1011(and11) autCA1012(A1012) mutants grown in Y-mannitol medium were analysed by SDS-PAGE.
When 20 μg of protein from conditioned medium was analysed, the separation between proMMP9 and MMP9 bands was lost and a large band of lysis was also seen at a size corresponding to MMP2.
The insulin content of the incubation medium was analysed using the high-range HTRF insulin kit (62INSPEC, Cisbio Bioassays, Codolet, France) and fluorescence resonance energy transfer was measured on an Envision plate reader (PerkinElmer, Cambridge, UK).
Matrix metalloproteinase (MMP) expression and activation in the medium were analysed by gelatin zymography, proteoglycan release by the dimethylmethylene blue assay, and cell viability by the Cytotox 96® assay.
