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After electrical stimulation as a quality test, cells were placed in a medium appropriate for the measurement of voltage- and store-independent Ca2+ influx containing diltiazem and ryanodine where Na+ was replaced by the large organic ion N-methyl-D-glucamine, as previously reported12 (Fig. S1).
The tumor cells were grown in medium appropriate for each cell line, supplemented with mitoxantrone (5 nM unless stated otherwise).
This makes this selective medium appropriate for tracing carbapenemase producers regardless of any resistance, by contrast to some other selective media.
After incubation at 35 °C for 45 min to 1 h, the viral inocula were replaced with serum-free medium appropriate for each cell line.
Bacteria were grown in a medium appropriate for the strain until an optical density of 0.8 to 1 at 600 nm was reached.
Stock solutions of 30% hydrogen peroxide, menadione sodium bisulphite and glucose/glucose oxidase (GO) (Sigma, Poole, UK) were diluted in medium appropriate for the cell line to be stressed.
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We used 1,2-dichlorobenzene (1,2-DCB) as a solvent which is one of the well-known mediums appropriate for dissolution of SWCNTs 34-35 withethe concentration of 0.1 mg/ml.
It is difficult to determine whether a specific medium is appropriate for development programmes.
On Day 1 of VEGF bioactivity experiments, microspheres were prepared by incubating Blank and 1.6% VBP, VBPWT, and Scramble microspheres at 1 mg mL 1 in 10 ng mL 1 VEGF and 0.1 wt % BSA in pH 7.4 PBS for 4 h and subsequently washed with 2, 10, or 25 vol % serum in basal medium as appropriate for each condition.
To assay for growth in phenotypic spotting assays, five-fold serial dilutions of saturated overnight yeast cultures grown in YPD medium, or in synthetic complete medium supplemented as appropriate for plasmid selection, were plated onto appropriate media at a starting OD600 of 0.5.
Swabs in bacterial transport medium are not appropriate for virus isolation or PCR testing.
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