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Upper-limit distance restraints of 2.7, 3.3, and 5.0 A were employed for direct NOE cross-peaks of strong, medium and weak intensities, respectively.
For resins with medium and weak basicity, large variation in the sorption capacity with concentration was observed.
The inter-proton distance restraints derived from the NOE intensities were grouped into three distance ranges, namely 1.8 2.9 Å, 1.8 3.5 Å and 1.8 6.0 Å, which corresponds to strong, medium and weak NOEs, respectively.
Inspired by the methodology of three-way decisions and protein tri-partition, this paper proposes a frequent pattern discovery algorithm for a new type of pattern by dividing the alphabet into strong, medium, and weak parts.
Different regions of strong, medium, and weak absorption are indicated in Figure 4.
We used trapezoidal membership functions (MFs) to represent low, high, very strong, and very weak and triangle MFs to represent moderate, strong, medium, and weak.
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NOE-derived interproton distances were sorted into classes in each NOESY spectrum, corresponding to approximate distance ranges: 1.8 3.0 Å (1 Å=0.1 nm) (strong), 1.8 3.5 Å (medium-strong), 1.8 4.0 Å (medium) and 1.8 5.0 Å (weak).
These intermolecular NOEs were classified as strong, medium, medium-weak, and weak according to the number of contour lines at the same threshold (Table 3).
The shades of the lines indicate the strength of the interactions observed: strong (dark blue), medium (blue), and weak (light blue).
Ten percent of invasive glioma cells showed strong staining for CTGF, 70% exhibited medium and 20% weak immuno-positivity while 80% of glioma cells in core samples exhibited weak staining and 20% exhibited medium staining.
The cross peaks are further categorized into 20 strong, 21 medium, and 24 weak restraints according to the relative intensity and the experimental methods (Supplementary Table 3).
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