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For growth tests on agar plates, cells were pre-grown on selective maltose-containing medium and washed two times with sterile water.
Micropatterned films of PLGA were removed from the growth medium and washed twice with PBS; cells were fixed in 4% paraformaldehyde and permeabilised with 1% Triton-X 100 solution (Applichem, Germany).
The cells were incubated for 4 h in serum-free medium and washed in PBS.
Cells were collected by trypsinization after treatment, suspended in a fresh medium, and washed with PBS two to three times.
Cells were then treated with trypsin, centrifuged at 1000 g, 5 min in culture medium and washed with PBS.
An equal volume of FCS was added following which the cells were resuspended in complete medium and washed four times.
Similar(31)
This will saturate the medium and wash out old fertilizer salts.
For cell culture, MC3T3-E1 cells were aliquoted at a cell density of 10,000 cells/well and then incubated for 2 days, followed by removal of the medium and washing with PBS.
After 12 h the drugs were removed by changing the medium and washing the cells once in medium.
Remove the cell culture medium and wash the cell monolayer with either pre-warmed HBSS or PBS and 1 mL of trypsin-EDTA to weaken cell adhesion to the flasks surface.
Remove medium and wash with PBS twice.
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