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It was larger than for any other medium and was perhaps the first time the figure has topped $70 billion.
Indeed, he almost landed in jail for contempt of court after exposing a fake spiritualist medium, and was only saved when the paper's crime correspondent pushed bundles of fivers into a police officer's hands.
The pigment was secreted in the medium and was also present intracellualry.
The adsorption equilibrium was influenced by the pH of the reaction medium and was unfavorable under acidic conditions.
Mixture of 5%% sodium alginate solution was poured into broth of basal salt medium and was sterilized.
A modified Pikovskaya medium (PVK) without yeast extract (Nautiyal 1999) was used as the culture medium and was added to each well in advance.
To evaluate the efficacy of the surface sterilization method the water used to rinse the tissues after surface sterilization was plated on selective medium and was incubated, too.
The effect of alcohol addition increased with increasing viscosity of the original medium and was most pronounced in highly viscous saccharose solutions (μL⩾100 mPa s).
The strain was grown at 37 °C with shaking at 200 rpm overnight, and then it was inoculated to the same medium and was grown to OD600 0.5–0.6.
Among the isolated strains, B47-9 was found to have the strongest ability to degrade PBSA and PBS films on agarose medium, and was the only strain that resulted to a detectable PBSA emulsion-degrading activity in liquid culture.
After the incubation, medium was discarded; the MTT (10 µL of 5 mg/mL) was added to 100 µL of fresh medium and was incubated for additional 4 h.
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