HUVECs were grown in 100-mm collagen-coated tissue culture plates in triplicates in EGM medium and treated with different doses of (FeCo Au for 2 h.
After the dye loading, the phytoplankton cells were rinsed, incubated with L1 medium, and treated with the 1 mg/ml TiO2, SiO2, and CeO2 respectively.
The microbial strains inoculated in appropriate growth medium and treated with different concentrations of Ag NPs were incubated for adequate time and optical density was recorded at 600 nm giving direct measure of microbial cell density.
Then, the medium was replaced with fresh medium and treated with CSSS, C-SLN and Tf-C-SLN (4 μM curcumin/well) using SS and B-SLN as the respective controls and incubated at 37 °C in CO2 incubator for 3, 6, 12, 24, and 48 h and the effect on cell viability was determined as described above.
Then, Hela cells were washed with PBS and incubated in a nanogel-free medium and treated with an 808-nm laser at 400 mW/cm2 for 15 min and a 680-nm LED lamp at 10 mW/cm2 for 40 min. For cell survival test, the irradiated plates were returned to the incubator, and cell viability was colorimetrically measured 48 h later with MTT assay [23].
Primary human MCs and LAD2 cells were cultivated in polyamine-free medium and treated with DFMO to block ODC activity.
Strains were grown to an OD600 of 0.8 in GM17 medium and treated as previously described [34].
Briefly, mid-log cell cultures were grown on appropriate medium, and treated or not treated with 0.5 M KCl for the indicated length of time.
The MDM cells were switched into fresh medium and treated with various concentration of rsTRAIL and/or AD5-10 [23] for a time course.
A549 cells were washed, diluted to 106/ml in serum-free medium and treated with HAMLET (36 µM) for 1 hour in 24-well culture plates (TPP, Trasadingen, Switzerland).
