HUVECs were grown in 100-mm collagen-coated tissue culture plates in triplicates in EGM medium and treated with different doses of (FeCo Au for 2 h.
After the dye loading, the phytoplankton cells were rinsed, incubated with L1 medium, and treated with the 1 mg/ml TiO2, SiO2, and CeO2 respectively.
The microbial strains inoculated in appropriate growth medium and treated with different concentrations of Ag NPs were incubated for adequate time and optical density was recorded at 600 nm giving direct measure of microbial cell density.
Then, the medium was replaced with fresh medium and treated with CSSS, C-SLN and Tf-C-SLN (4 μM curcumin/well) using SS and B-SLN as the respective controls and incubated at 37 °C in CO2 incubator for 3, 6, 12, 24, and 48 h and the effect on cell viability was determined as described above.
Strains were grown to an OD600 of 0.8 in GM17 medium and treated as previously described [34].
Primary human MCs and LAD2 cells were cultivated in polyamine-free medium and treated with DFMO to block ODC activity.
Control (MAA treatment for 24 h followed by recovery in normal culture medium) and treated cells were analyzed as described below.
Briefly, mid-log cell cultures were grown on appropriate medium, and treated or not treated with 0.5 M KCl for the indicated length of time.
The different mycobacterial strains were grown in Sauton's medium and treated with varying concentrations of TAC or its analogues as discussed in the text.
The MDM cells were switched into fresh medium and treated with various concentration of rsTRAIL and/or AD5-10 [23] for a time course.
For double thymidine cell arrest, cells were treated with thymidine (Sigma) at 2 mM final concentration for 18 hours, released in normal medium, and treated again for 18 hours with a 2 mM final concentration of thymidine.
