Sentence examples for medium and supplements from inspiring English sources

All cell culture medium and supplements were obtained from Lonza BioWhittaker (Walkersville, USA) unless otherwise stated.

Culture medium and supplements including serum were from BioWhittaker (Walkersville, MA).

Endothelial cell growth medium and supplements were obtained from ScienCell Research Laboratories (San Diego, CA).

SB were then re-cultured with fresh serum-free medium and supplements in low adherent dishes.

PC3 and DU145 were cultured in RPMI 1640 (Invitrogen) medium containing 5 mM L-glutamine and fetal bovine serum (FBS) and 1542-NPTX and 1542-CP3TX were maintained in Keratinocyte-SFM (KSFM) medium and supplements (Invitrogen) with 5% FBS and KSFM supplements (Invitrogen).

Culture medium and supplements were obtained from Invitrogen (Renfrewshire, UK).

Medium was replaced directly at D7 with defined keratinocyte serum-free medium and supplement (DSFM) to the confluent cell layer.

48 hours later, the virus-containing medium (10 ml) was filtered, mixed with 5 ml of freshly prepared medium and supplemented with 4 µg / ml polybrene.

In stage 3, cells were cultured in 2% FBS/DMEM (25 mM glucose)/F12 medium and supplemented with 10 mM nicotinamide and B27 (GIBCO) for 7 days.

In stage 4, differentiated premature islet-like cell clusters were cultured in stage 3 medium and supplemented with SCM for 14 days.

Supernatants were diluted 1 1 with target cell line normal growth medium and supplemented with 8 µg/ml polybrene (Sigma).