Sentence examples for medium and supplemented from inspiring English sources

A BR 15.4 bioreactor (B. Braun Melsungen AG, Germany) filled with 10 L YMG medium and supplemented with 0.5 % talcum powder (Sigma-Aldrich, St . Louis USA) was inoculated with 100 mL of the seed culture.

Each well of a 96 well microtiter plate was filled with 130 μl sterile autotrophic and heterotrophic broth medium and supplemented with different concentrations of As III) as NaAsO2 (0–30 mM) Strains were grown in 5 ml of their respective autotrophic and heterotrophic broth medium without arsenic for 48 72 h at 28 ± 2 °C on a rotary shaker (150 rpm).

HL-60 cells were obtained from the NCCS, Pune, India, and grown in RPMI1640 medium and supplemented with a 2 mg mL−1 sodium bicarbonate, 4.5 mg mL−1 glucose, 100 μg mL−1 streptomycin sulfate, 40 μg mL−1 gentamycin, 100 U mL−1 penicillin as well as 10%% heat-inactivated fetal calf serum.

To induce cell suspension cultures, 2 g fresh weight (FW) of friable callus was transferred to 250-mL Erlenmeyer flasks containing 40 mL MS liquid medium and supplemented with sucrose (30 g/L) and growth hormone of NAA (2 mg/L) and kinetin (0.1 mg/L).

In stage 3, cells were cultured in 2% FBS/DMEM (25 mM glucose)/F12 medium and supplemented with 10 mM nicotinamide and B27 (GIBCO) for 7 days.

In stage 4, differentiated premature islet-like cell clusters were cultured in stage 3 medium and supplemented with SCM for 14 days.

THP-1 cells were cultured in RPMI 1640 medium (Gibco) and supplemented with 10% FBS (Hyclone Lab Inc).

Parental and DNMT KO HCT116 cell lines were grown in McCoy 5A medium (Sigma) and supplemented with 10% FBS.

Cells were subject to half-medium changes and supplemented with fresh cytokines every 48 h.

After 48 h, the virus-containing medium was filtered and supplemented with 8 µg/ml polybrene.

Medium was replaced and supplemented with the investigated substance.