Sentence examples for medium and supplement from inspiring English sources

Medium was replaced directly at D7 with defined keratinocyte serum-free medium and supplement (DSFM) to the confluent cell layer.

Medium and supplements were from Sigma.

Cells were cultured using appropriate medium and supplements recommended by the suppliers.

The hiPSC-CM were cultured in RPMI Medium and supplemented with 2% B-27 minus insulin (Gibco).

All cell culture medium and supplements were obtained from Lonza BioWhittaker (Walkersville, USA) unless otherwise stated.

Culture medium and supplements including serum were from BioWhittaker (Walkersville, MA).

Endothelial cell growth medium and supplements were obtained from ScienCell Research Laboratories (San Diego, CA).

SB were then re-cultured with fresh serum-free medium and supplements in low adherent dishes.

48 hours later, the virus-containing medium (10 ml) was filtered, mixed with 5 ml of freshly prepared medium and supplemented with 4 µg / ml polybrene.

In stage 3, cells were cultured in 2% FBS/DMEM (25 mM glucose)/F12 medium and supplemented with 10 mM nicotinamide and B27 (GIBCO) for 7 days.

In stage 4, differentiated premature islet-like cell clusters were cultured in stage 3 medium and supplemented with SCM for 14 days.