Sentence examples for medium and stained from inspiring English sources

Animals were washed 5 times with M9 medium and stained with 2 µM SYTOX™ Green (Invitrogen) for 5 minutes at room temperature [48].

U937 cells were washed in serum-free medium and stained with the membrane dye PKH26 (Sigma), according to the manufacturer's instructions.

The cell suspension was filtered through a 40 μm filter unit (Fisher), centrifuged and resuspended in up to 0.5 ml staining medium, and stained with FDG using FluoReporter LacZ Flow Cytometry kits (Molecular Probes, F-1930) following the manufacture's instruction.

Negative controls were glioblastoma cells showing mesenchymal phenotype but not exposed to adipogenic medium; positive controls consisted of marrow stromal cells exposed to adipogenic medium and stained with Oil Red-O.

The day after the transfection, the cells were diluted 1 6 and, starting from the day after, selected for 6 weeks by adding 0.4 mg/ml G418 to the culture medium and stained with 0.3% crystal violet in 30% ethanol.

Target EL4 cells were incubated in the absence or presence of the OVA peptide for 1 h at 37°C, rinsed once in serum-free medium, and stained with the PKH26 dye using PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich).

Detection of growing rickettsiae was monitored by using Gimenez staining and an immunofluorescence assay of cells collected after centrifuging the medium and staining with rabbit antirickettsial hyperimmunserum and Alexa Fluor 488 goat antirabbit immunoglobulin (Ig) G (H+L) conjugate (Invitrogen, Carlsbad, CA, USA) as secondary antibody.

CLSM analyses showed that only a few lipid vacuoles were present in MDA-MB-231 cells cultured in complete medium (CTR) and stained with Bodipy 493/503 (green), a fluorescent hydrophobic molecule that selectively localizes to neutral lipid aggregates [ 33].

HCT116 tumor cells and CD40 Bs from a healthy HLA-A02+ donor were washed in serum-free RPMI 1640 medium, pelleted and stained with 5 μM 5-chloromethylfluorescein diacetate or 5 μM 5- and 6)-(((4-chloromethyl) benzoyl)-amino) tetramethyl-rhodamin in PBS, respectively.

Attached cells and cells in the medium were collected and stained using ethidium homodimer 1 dye for dead cells and calcein AM dye for live cells.

CPC were also cultured for four weeks with osteogenic medium and were stained with von Kossa and for alkaline phosphatase to investigate the osteogenic potential.