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The cell pellet was diluted with 1-mL medium and spread into a new cell culture flask containing 20 mL Ham's F-12 medium with additives.
In our study, ZnO nanoparticles that are dispersed in the culture medium and spread over the cell surface could only enter the cells via their apical surface.
The respiratory samples were already diluted in virus transport medium and spread onto the cell monolayer.
HUVEC cells were resuspended in 1× Ringer medium and spread on 10 µg/µl fibronectin coated glass slides.
For induction and regeneration of apt mutants one-day-old protonemata were after Bleomycin treatment thoroughly rinsed with H2O, suspended in 2 mL of BCDAT medium, and spread on cellophane overlaid BCDAT agar plates, which were for selection supplemented with 2-FA (2-Fluoroadenine, Sigma-Aldrich, cat. Nr. 535087) and further incubated in growth chamber.
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Approximately 0.1 mL of each of the other samples was placed directly onto a 7H10 Middlebrook medium plate and spread over the plate by using a sterile loop.
The novela originated more than 50 years ago in Mexico as a radio melodrama, but when television appeared the genre was quickly adapted to the new medium and soon spread all over Latin America.
For HR and NHEJ assays, cells were grown in pre-induction medium (raffinose medium) and then spread on plates containing either galactose or glucose.
Cultured parasites were washed twice in SDM79 medium without serum and spread directly onto poly-L-lysine coated slides.
There were small, medium, and large mines spread throughout the surveyed basins.
VSMCs, 48 h post-transfection, were detached and transferred to glass coverslips with fresh culture medium and allowed to spread for 24 h.
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