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Negative controls were equivalent volumes of complete binding medium and serum from a non-immunised rabbit.
Cell culture medium and serum were obtained from Lonza Benelux B.V. (Breda, The Netherlands) and penicillin-streptomycin and trypsin were from Invitrogen (The Netherlands).
Medium and serum were purchased from Invitrogen (Carlsbad, CA).
Cells were rinsed with serum-free medium, and serum starved for 24 hours.
HEK293T (293T) and GC-2spd ts) GC-2spd ts obtained from the Americellsype Culture Collection (ATCC) and cultured were the medium and serum as ATCC recobtained.
During the initial monolayer culture, chondrocytes are extracted from their normal 3D environment and exposed to an artificial environment consisting of a plastic surface, culture medium and serum.
Similar(48)
For cell apoptosis analysis, cells were cultured overnight with both serum-containing complete medium and serum-depleted medium; the attached cells and floating cells were then harvested.
We used this selectivity difference to design amino acid determinations of diluted cell culture and fermentation broth media, including Bacto yeast extract peptone dextrose (yeast culture medium) broth, Luria Bertani (bacterial culture medium) broth, and minimal essential medium and serum-free protein-free hybridoma medium (mammalian cell culture media).
After comparing the migration pattern of 3 control and 3 FD hOE-MSCs in serum medium and serum-free medium (ITS), we determined that FD cells invasion is significantly reduced compared to control cells both in serum and in ITS medium (Figure 5).
Interestingly, consequences of incubation in normal medium and serum-free medium were similar but not identical.
All U251 cell culture supernatants were separately collected as serum conditioned medium and serum-free conditioned medium.
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