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104 labelled cells (MIRB-MSCs and Cell Sense-MSCs) were resuspended in culture medium and seeded on a chamber slide.
Primary rat MSCs were harvested from bone marrow, cultured in dexamethasone containing medium and seeded directly onto the scaffolds.
After 80%% confluence, HUVECs were suspended in complete medium and seeded onto the various substrates at a concentration of 5 × 104 cells per well.
In all experiments cells were suspended in the growth medium and seeded in 24-well plates at 2 × 105 cells/well.
The cells were adjusted to a concentration of 2 × 106 viable cells/ml in RPMI 1640 growth medium and seeded in 96-well tissue culture plate @ 100 µl/well.
The human monocytic leukemia cell line THP-1 (floating monocytes, 500 000 cells/mL) was incubated in 100 μL of the serum-free RPMI 1640 medium and seeded into 96-well plates in triplicate at 37°C.
Cells were diluted in fresh complete medium and seeded in 96-well plates.
Peritoneal cells were harvested by peritoneal lavage with complete RPMI medium and seeded at 1×106 cells/ml into tissue culture dishes.
After mechanical detachment (check-off) cells were washed three times in PBS, one time with complete medium and seeded in a new dish.
The transfected cells were then suspended in an appropriate volume of 20% FBS supplemented DMEM-LG medium and seeded for further culture.
Labelled outer segments were washed, resuspended in human foetal RPE medium and seeded onto iPS-RPE cells cultured on gelatin-coated 35 mm dishes.
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