Sentence examples for medium and samples from inspiring English sources

After the second block, cells were released into fresh medium and samples were collected either for cell cycle analysis using propidium iodide staining followed by Fluorescence Activated Cell Sorting (FACS) analysis, or for whole cell extract (WCE) preparation.

After 24 h, complete medium was removed and renewed with hormone-free low serum (0.5% CS-FBS) medium, and samples incubated for 24 h in order to starve HUVECs to achieve a quiescent state.

Avoid cooling of medium and samples.

ARA (1 mM) was added to the medium and samples were taken at different time points.

Avoid cooling of medium and samples. 5. Aspirate off the medium and rinse cells once with 1 × PBS.

Cells were grown in YEPD medium and samples were taken for flow cytometry as previously reported (Li et al. 2013).

The Ca2+-free RBCs above the oil and the pelleted dense cells still containing Ca2+ were harvested into microfuge tubes by gentle resuspension in 0K medium and sampled for Hb to estimate their yields.

Fold-change calculations for time course expression were undertaken pair-wised between samples of 4 hours in CENBA medium and sample at 24, 48, 72 and 96 hours in CENBA medium using Cuffdiff with geometric library normalization method and minimum alignment counts of 10 [ 74, 75].

Skin samples cultured in medium only and samples co-cultured with lymphocytes from a "syngeneic MLR" were used as controls.

Finally, the slides were mounted with DAPI (4,6-diamidino-2-phenylindole) mounting medium (Sanda Cruz Biotechnologiesamplessamples were visualized with a Zeiss Axiovert microscope using filters at 488, 546 and 340/360 nm.

Medium and biomass samples were taken as previously described, with an additional sampling of mycelium from 10 ml broth volume for enzymatic assays.