Sentence examples for medium and sampled from inspiring English sources

The Ca2+-free RBCs above the oil and the pelleted dense cells still containing Ca2+ were harvested into microfuge tubes by gentle resuspension in 0K medium and sampled for Hb to estimate their yields.

To assess the effect of either phb-1 or phb-2 deficiency on the whole worm GC/FID and H NMR profiles, synchronous populations of N2 wild type worms (approximately 2 worms/μl) were grown in liquid medium and sampled (approximately 30,000 worms) at either L4 larval or young adult (YA) stages.

At the completion of each experiment, the porous medium was extruded and sampled directly for cell retention on the basis of a radiolabel mass balance.

Control and Wee1 siRNA-treated cells were synchronized at pro-metaphase, released into the taxol-containing fresh medium (time 0) and sampled at indicated time points.

A culture of asci was exposed to growth medium and subsequently sampled to determine the antibiotic resistances of the sample colonies.

After 24 h, complete medium was removed and renewed with hormone-free low serum (0.5% CS-FBS) medium, and samples incubated for 24 h in order to starve HUVECs to achieve a quiescent state.

After the second block, cells were released into fresh medium and samples were collected either for cell cycle analysis using propidium iodide staining followed by Fluorescence Activated Cell Sorting (FACS) analysis, or for whole cell extract (WCE) preparation.

Avoid cooling of medium and samples.

ARA (1 mM) was added to the medium and samples were taken at different time points.

Avoid cooling of medium and samples. 5. Aspirate off the medium and rinse cells once with 1 × PBS.

Serial 10-fold dilutions were then prepared in the same medium and samples (0·1 ml) were plated on selective media.