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After incubation for 30 min, the matrix was flushed with Hanks medium and removed.
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Reduce heat to medium, and remove skate to a warm platter.
The left lateral and medium lobes were ligated and removed.
Planktonic cells and medium were removed and wells were washed four times with 200 µL H2O.
After 3 and 6 h, medium was removed and cells were washed 3 times with PBS.
Flasks were cooled on ice, and culture medium removed and cell layers washed with phosphate buffered saline.
2. Start blender on medium-high speed and remove center of lid.
The medium was removed and treated with fresh medium containing PLGA NP, PCC, and FPCC.
The old medium was removed and subsequently the fresh medium was added with 100 μL/well.
Then, the medium were removed and replaced with 100 μL fresh culture medium.
The infectious medium was removed and replaced with fresh medium 4 h after infection.
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