Sentence examples for medium and recovered from inspiring English sources

Cells were resuspended in 1 ml of SOB medium and recovered for 1 h at 30 °C.

MPs were incubated with dispase for 2 hours at 37°C, then homogenized and centrifuged at low speed for 10 minutes, washed several times in PBS, resuspended in fresh RPMI medium and recovered cells were counted using a hemocytometer.

MDA-MB-231 cells were cultured without refreshment of culture medium and recovered for several rounds.

Cells were re-suspended in 1 ml of SOC medium and recovered for 1 2h at 37°C.

Following chromatographic separation of free and incorporated SO4-2 using PD-10 columns (Pharmacia, Piscataway, NJ, USA), radiolabeled GAGs released into the culture medium and recovered by alkaline extraction were quantified using scintillation counting.

The voltage was 1.5 kV, and the time constant was 5 ms. The electroporated cells were transferred to 10 ml of complex modified VM medium, and recovered for 24 h at 34°C.

Therefore, the possibility of removing 12C from the fresh medium, and recovering and recirculating the inorganic carbon in the supernatant and the organic carbon from the EPA depleted biomass was studied.

The amount of ET/QD entrapped in NPs was estimated by suspending 10 mg of NPs in aqueous medium and recovering the NPs pellet by centrifugation for 30 min at 20,000 rpm (Sigma 3 K30, Germany).

The Huh-7 was maintained at - 80°C in Dulbecco's Modified Eagle Medium (DMEM) and recovered from preservative by centrifugation.

Proliferating cells could be frozen down in 10% DMSO in proliferation medium and be recovered by rapidly bringing them to 37°C and plating in fresh medium (not shown).

Next day, the cells were transfected with NF-κB reporter plasmid of NF-κB-TA-luc (Clontech, San Diego, CA) by using Lipofectamine 2000 in 10% FBS Opti-MEM medium for 16 hours, and recovered in fresh complete RPMI medium for additional 12 to 16 hours.