Sentence examples for medium and protein from inspiring English sources

The yeast Saccharomyces cerevisiae is suitable as host for production of peptide library; this is due to their abundance in gene manipulation tools, fast growth in a low-cost medium, and protein folding and secretory machinery homologous to that of mammalian cells (Idiris et al. 2010).

Cells harboring Snl1 or its derivatives were induced in galactose medium, and protein expression was monitored in whole-cell lysate, supernatant, and pellet.

In all cases, E. coli cells carrying the recombinant pET24b-BCO plasmids were cultivated in LB medium and protein expression was induced by 1 mM IPTG addition.

Approximately 0.5 mL overnight culture was inoculated into 50 mL LB medium, and protein expression took place under different induction conditions (Table 2).

The colony carrying the recombinant plasmid was grown in LB medium and protein expression was induced by 0.5 mM isopropyl-β-D-thiogalactopyranoside at 16 °C for 16 h.

Then 0.4 mM δ-aminolevulinic acid was added to the medium, and protein expression was induced with 1 mM isopropyl β- d-1-thiogalactopyranoside. Protein expression was continued for 20 24 h at 37 °C.

Surface wettability is believed to be an important factor that guides the first events occurring at the cell/biomaterial interface, such as interaction of medium and proteins with biomaterial and subsequent cell responses [34].

The day after the transfection, metformin was added to the medium and proteins were extracted 96 h after the addition of metformin.

GFP+ and GFP− cells were positive for high, medium, and two low abundance transcripts (Actb, high; γ-actin or Actg, medium; Odc and protein phosphatase 1cα or Ppp1ca, low), indicating good amplification.

By use of mass balances that involve the protein initially added to the synthesis medium, and the protein remaining in the supernatant and washing solutions (these last derived from activity measurements), the precipitable protein present in CLEAs is obtained, and their specific activity can be calculated.

Total milk yield, milk fat and milk total solids, and weaning weight, average daily gain and relative growth rate of lambs were higher (P < 0.05) for ewes fed medium and high protein levels compared to those fed low protein level.