The medium and procedures for seed culture and chemostat cultivations were described in Nie et al. (2014) with glucose as the only carbon source.
The tissue that was not completely disrupted was re-suspended in the medium and the procedure above was followed, for a maximum of 3 times.
For the functional test, medium and thawing procedure (e.g. addition of DNAse, of a resting phase, etc).
Bernardi et al. in their survey showed the correlation between radiation dose and types of interventional cardiology procedures with mean DAP being 65.8 Gy × cm, 93 Gy × cm, and 116.7 Gy × cm, corresponding to the simple, medium, and complex procedure groups [ 79].
Two factors for secondary caries formation were evaluated using an artificial biofilm model, saliva as additive in culture medium and bonding procedures of composite materials for artificial gap creation.
The use of artificial growth medium and extraction procedures [6] neglects the importance of the ecological functions of soils.
DNA extraction from 200μl 2-SP transport medium and PCR procedures were performed according to the manufacturer's instructions.
During 1979, the composition of the selective medium and the laboratory procedure (inoculation onto one vs two media per specimen) were changed (table 1).
7 mL of 0.25% trypsin solution was added to the remaining tissue mash in the trypsinization flask and stirring and incubation was repeated for 7 min. Thereafter, the supernatant was effused again into a new 50 mL tube filled with washing medium and the whole procedure was repeated.
Chloroplast transformants were identified by growth on selective medium and PCR (Experimental Procedures).
