For transcriptome sequencing, fungal mycelia were collected at 40 hours and 72 hours for each medium and were preserved in TRIzol® Reagent (Invitrogen).
The DRGs were extracted from the mouse at E13 (Embryonic day 12) or E14 and preserved in medium (containing serum).
Salivary glands were obtained by dissection under a zoom stereomicroscope (3X magnification; Nikon SMZ800, Japan) and preserved in RPMI 1640 medium (GIBCO BRL) or PBS.
Biotin was detected with an ABC kit (Vector Laboratories) and visualized by incubation in DAB for 3 min. Sections were slide-mounted, dehydrated and preserved using Permount mounting medium.
SB15006 and SB15007 strains were grown on ISP4 medium for sporulation, and spores were harvested and preserved as a 20% glycerol suspension at −80 °C.
Stock cultures were maintained on Reinforced Clostridial Medium (RCM, Oxoid Deutschland GmbH, Wesel, Germany) and preserved using glycerol 20%% (v/ v) at −80 °C.
Though microfilm would remain an important medium for storing and preserving documents, it proved too unwieldy, too fragile, and too expensive to serve as the basis for a broad system of knowledge transmission.
After 24 h cultivation, 40 mL medium was taken and cells were harvested by centrifugation at 10,000 rpm for 15 min and preserved at 4°C in refrigerator.
"But we think there is a chance to produce a new kind of film and preserve this medium".
For Thomas, the biggest question will always be: "What should a modern Today programme sound like?" Radio, he says, "is a medium of connection, of identification, and preserving that is key.
