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The various concentrations of nanoparticles, 50, 100 and 200 μl, were mixed with the medium and poured in Petri plates.
The transformation mix then was mixed with 150 mL of regeneration medium and poured into 20 Petri dishes (7.5 mL dish−1).
Nutrient agar was inoculated with microbial cell suspension (200 μl in 20 ml medium) and poured into sterile petri dishes.
To make a Thermotoga plate, the liquid culture was mixed with hot SVO Gelrite medium and poured into petri dishes.
The sterile nutrient agar was inoculated with microbial cells (200 μl of microbial cell suspension in 20 ml agar medium) and poured into sterile petri dishes.
After 6 h of incubation at 77°C with gentle agitation (100 to 125 rpm), the culture was mixed with hot SVO Gelrite medium and poured into petri dishes, with a supplement of 250 μg/ml kanamycin for selection.
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As soon as it turns medium brown, turn down the heat to medium and pour in the ginger paste.
When all the meat is in the casserole, turn the heat under the frying pan to medium and pour in the wine to deglaze, scraping up any caramelised bits.
Sterile potato dextrose agar medium was prepared and poured into sterile Petri plates and allowed to solidify; after solidification, fungal cultures were swabbed on these plates.
3. Melt butter in maple syrup over medium heat, and pour over turkey.
Place the gelatin in a medium bowl and pour in 1/4 cup of the water.
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