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The Happel model was used to calculate the resulting permeability values in order to study the interaction of k with gel mechanical properties and mesenchymal stem cell (MSC -induced gel contraction, MSC -inducednd differentiation in both non-osteogelicontractiondiumetabolismeogenic medium for up to 3 weeks.
These findings are strengthened by the results obtained for the cells cultivated with stem cell medium and osteogenic medium for 4 and 12 days.
The differences appeared between stem cell medium and osteogenic differentiation medium, as inducing the osteogenic differentiation had caused, as expected, a decrease in cell numbers after 4 and 12 days of cultivation (Additional file 4: Figure S4).
DF stem cells cultivated in stem cell medium and osteogenic differentiation medium OS and OC showed that the type of implant do not influence the cell viability and proliferation.
In this study, we testified our hypothesis and elucidated that the opposite effect of canonical Wnt signaling in the fully cultured medium and osteogenic differentiation medium was mainly caused by different expression level of miR-17.
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Two types of culture medium, i.e. growth medium (GM) and osteogenic medium (OM), were evaluated.
ADSCs at a density of 1 × 106/cm2 were cultured in the osteogenic medium and the osteogenic cell sheets could be obtained after 14 d.
(A) Mean time-course Raman spectra of typical 210 × 210 mm2 regions of the cultures shown in C. Raman spectra were acquired at different time points for the same regions of the culture osteoblast in osteogenic medium and non-osteogenic medium.
The hMSCs were differentiated into osteoblasts using Osteogenic differentiation media which comprises of Osteogenic Differentiation BulletKit® that contains Basal Medium and one Osteogenic SingleQuot Kit® also purchased from Cambrex Bio Science (Walkerville, MD).
Titanium surfaces coated with hydroxyapatite in the presence of stem cell medium and complex osteogenic medium proved to be the most favorable in terms of Ca2+ deposition on the implants' surface (Fig. 9b).
Following methods were used to compare glioblastoma cells and GFAP+NNP (NHA): exposure to neural differentiation medium, exposure to adipogenic and osteogenic medium, western blot analysis, immunocytochemistry, single cell assay, BrdU incorporation assay.
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