Exact(3)
At 24 hours of incubation, the amount of lactate accumulated in the medium and normalized to 1 million cells revealed four folds higher productivity by the strain synthesizing Fragment 9 with respect to the strain encoding the native PFK-M and six folds higher in comparison to the parental strain.
Supernatants were vacuum-dried, resuspended in RPMI medium, and normalized by volume to OD600 = 1.5.
Data were calculated according to the amount of medium and normalized to the number of cells or cartilage wet weight (monolayer or tissue explants, respectively) and control group, which was set at 100%.
Similar(5)
Rates of gas production were calculated from a linear regression over the entire batch cycle and normalized by medium volume.
The specific strains studied were HI2424 and AU1054, which were grown in both synthetic CF sputum medium and soil medium (SE); the normalized results for SE conditions for both strains were obtained as it likely represents a more natural growth condition.
Expression levels of Fas1 and Fas2 genes of the WT C. parapsilosis grown in glucose and fatty acids containing medium were normalized to WT grown in YP medium (1% yeast extract, 2% peptone).
The total amounts of the FGF21 in medium were normalized to the total protein amounts and reported as pg/mg protein.
Free glycerol levels present in the medium were determined using a colorimetric kit (Free Glycerol reagent; Sigma-Aldrich) and normalized to cell density.
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