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The TiAlSiCN coating was the only one which sustained repetitive impact load for 105 cycles in air (maximal normal load of 1000 N), as well as in distilled water (neutral medium) and normal saline (corrosive medium) at 500 N.
An automatic injector (CT Injector Missouri, Ulrich Medical) was used for injection of the contrast medium and normal saline.
The conditioned medium (CM) was composed out of cell growth medium and normal DMEM medium in a ratio of 1 : 2 on respective cell type.
The used conditioned medium (CM) was composed out of cell growth medium and normal DMEM medium in a ratio of 1 : 2 on respective cell type.
In accordance to (1), a linear dependence of the solubility CH is observed for each series of measurements, in which three different supersaturation values (zero, medium and normal) have been established.
All transformants carrying empty plasmid or Snl1 under native Snl1 promoter showed white colony color phenotype when grown on limiting Ade medium and normal growth on medium lacking adenine (data not shown).
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We categorized phenotype severity into four different classes: severe, medium, mild and normal (Fig. 1F-I).
A cranial computed tomography scan was performed (without contrast medium), and was normal apart from calcificated basal nuclei as a sign of old inflammatory episodes.
For each strain, at least four different clones were sequenced, each clone deriving from a single colony grown on solid medium and under normal growth conditions.
After a 3-hour incubation at 37°C, the medium was aspirated, and normal growth medium was added.
Cells were exposed to drug for 24 hours, and then drug-containing medium was aspirated and normal culture medium was added.
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