The formulation of the TAP media, A+ medium, and modified derivatives of each can be found in the supplemental data (Additional file 1: Table S1).
RPMI 1640 medium and modified RPMI 1640 without choline, serine, methionine, inositol and folic acid (called modified RPMI in the text) were obtained from Gibco (Saint Aubin, France).
African green monkey kidney (Vero E6) cells, baby hamster kidney (BHK21) cells and 293T cells were grown and maintained in Dulbecco's modified Eagle medium and modified Eagle medium (Gibco Invitrogen), respectively, supplemented with 10% heat-inactivated fetal bovine serum and 100 U/ml of penicillin and 100 µg/ml of streptomycin (Gibco Invitrogen).
Two media were used to culture Leishmania spp: Novy-MacNeal-Nicolle (NNN) medium with Offutt modification and modified NNN medium with Roswell Park Memorial Institute medium overlay (11 ).
The electrooxidation of d-glucose has been studied in alkaline medium on pure and modified noble metal electrodes.
Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified eagle medium as negative control, modified eagle medium with 25 μg/mL EMD, modified eagle medium with 100 μg/mL EMD, and modified eagle medium plus 10% fetal bovine serum as positive control.
U937 cells and A549 cells (American Type Culture Collection) were grown in RPMI-1640 medium and Dulbecco modified eagle medium, respectively, containing 10% fetal bovine serum and 2 mM l-glutamine.
Many of the processes occurring during capacitation can be recapitulated in chemically defined solutions, e.g. Biggers, Whitten and Whittingham medium (Biggers et al., 1971) and modified tyrodes (mT6) medium (Fleming et al., 1986) thus facilitating a dissection of the signalling cascades within sperm.
To evaluate the efficacy, tolerability, and compliance of 3 ketogenic diets, the classical ketogenic diet, medium-chain triglyceride (MCT), and modified Atkins diet.
The newly isolated fungal strain Aspergillus niger ADH-11 produced a maximum of 22.14 U/g of α-L-arabinofuranosidase under solid-state fermentation using wheat bran as the substrate and modified Mandels-Weber medium at 30°C after 180 h of incubation.
Water samples; swab samples; and spleen, liver, and bone marrow homogenates were cultured on cysteine heart agar supplemented with 9% sheep blood, Columbia blood agar, McConkey agar, and modified Thayer-Martin medium containing antimicrobial drugs (Merck, Darmstadt, Germany).
