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The production of CphEal was assayed in complex LB medium and minimal M9 medium with 2% (vol/vol) glycerol.
This design was performed in two medium types: rich YM medium and minimal medium, based on date juice (Yucca medium).
It was cultured in RPMI 1640 medium and minimal essential medium supplemented with 10%% fetal calf serum, 100 U/ml penicillin, and 100 μg/ml streptomycin, 4 mM l-glutamine under 5%% CO2, and 95%% humidified atmosphere at 37 °C.
Three cases representing a maximum hazard day (quiet winds <1 m s−1 near the wellhead) and medium and minimal hazard days (average winds 3 m s−1 and 7 m s−1) were assessed.
Yeast minimal SD base medium and minimal SD agar base were purchased from Ozyme (Saint-Quentin-en-Yvelines, FR).
To validate our in silico data and test whether autophagy could be induced by nutrient limitation in P. falciparum, parasite cultures were grown in both serum-free medium and minimal medium devoid of the essential amino acid L-glutamine and serum, with minimal glucose.
Similar(54)
We used this selectivity difference to design amino acid determinations of diluted cell culture and fermentation broth media, including Bacto yeast extract peptone dextrose (yeast culture medium) broth, Luria Bertani (bacterial culture medium) broth, and minimal essential medium and serum-free protein-free hybridoma medium (mammalian cell culture media).
Strains with different genotypes regarding pdhR were grown in rich medium (LB) and minimal medium (MM) supplied with glucose, glycerol, acetate or pyruvate as carbon source.
To test a putative role of Eng2 for morphogenesis, ENG2 was deleted using the strategy outlined in Fig. 5. Growing the deletion strain in various media (1% yeast extract with 3% glucose, Sabouraud's medium, RPMI, and minimal medium) in solid and liquid cultures at different temperatures did not show a growth phenotype.
Phosphate-buffered saline (PBS; 10 mM, pH 7.4), Dulbecco's modified Eagle medium (DMEM), and minimal essential medium (MEM) were purchased from Gibco (Life Technologies Corporation, Carlsbad, CA, USA).
Cells were grown in Dulbecco's modified Eagle's medium (DMEM) and Minimal essential medium with Earle's salt (EMEM), respectively, and supplemented with 10% fetal calf serum (FCS) and maintained in the appropriate medium with 1% FCS.
Related(20)
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