Investigators must remember to consider biology, biologic medium, and laboratory methodology when specifying a statistical model and its underlying assumptions appropriate for study.
When rhTyrRS was expressed in E. coli strain BL21 using M9CA medium and fed-batch methodology, approximately 50% of the protein was soluble.After 6 h of IPTG induction, cells were harvested by centrifugation and lysed by two cycles of microfluidization.
Quartz sand is utilized as a model medium, and the applicability of the methodology to determine radionuclide transport in engineered backfill cement is explored using the UK GDF candidate backfill cement, Nirex Reference Vault Backfill (NRVB), in a model system.
In practice, no high throughput or even medium throughput methodology has been developed for this and this is now the rate-limiting step in the identification of further genes that cause disease.
Full results and methodology.
See full results and methodology.
"My principle of work is based on training and methodology: to have the right training methodology.
Modelling theory and methodology.
One important dimension involves disciplines and methodology.
First, wording and methodology matter.
