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He began pushing at the limits of his medium and later said that he threw out most of two years of work.
The consortium was initially grown in acetate containing medium and later acclimatized to utilize CO as the sole carbon source for hydrogen production.
HIC on Butyl 650s (50 mM ammonium acetate pH 5.5 and 1.5 M ammonium sulphate) absorbs GOx from the medium and later it is eluted by 100% stepwise gradient with salt free buffer directly into SEC column (Sephadex 200) for desalting and final polishing separation.
Glucose was autoclaved separately from the minimal medium and later added to the media at the concentration of 20 g/L.
The total amount of methanol is the same in both reaction mixtures, but in the latter, the LaPO4 NCs were suspended in apolar medium and later methanol was added to the reaction mixture.
A major revision separated Am (meaning originally medium and later monsoonal) from Aw, dependent on whether the minimum monthly rainfall (< 60 mm/month) is greater than 4% of the annual rainfall or not.
Similar(46)
The 9.45-inch heavy mortar equipped a heavy trench mortar battery, while medium trench mortar batteries were equipped with the 2-inch mortar mortand and later the 6-inch mortar.
Briefly, VLPs were preincubated with serum-derived purified HBV (ID326, genotype D) at a ratio of 10 HBV genomes/hepatocyte for 1 h at 16 °C in Tupaia hepatocyte medium (THM) and later incubated on primary Tupaia belangeri hepatocyte cultures (PTHs) for 16 h at 37 °C.
Then, 5 h after transfection, 10% FBS was added to the medium and 2h later 50 µM 2-BP was also incorporated.
The verb started in the 13th century with the meaning of "to guide along a pathway or though a certain medium" and much later found a home in "the transmission of electrical signals".
Indeed, pure brain EC populations were isolated following serial incubation of human iPS or ES cells first with medium favoring neural differentiation and later with medium favoring endothelial differentiation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com