Sentence examples for medium and labeled from inspiring English sources

For in vivo labeling with 32P, cells were washed 3× with phosphate free medium and labeled with carrier free 32P-orthophsophate exactly as described before [35].

These monocyte-depleted lymphocytes were washed (3×) and resuspended in serum-free XVIVO-10 medium and labeled with CFSE following the instructions of the manufacturer (CellTrace CFSE cell proliferation kit, Molecular Probes, Paisley, UK).

In brief, cells were harvested from culture, washed with serum-free RPMI medium, and labeled with 0.5 μmol/L Texas-red ceramide for 30 minutes at 4°C.

Briefly, endodermal cells derived from the ventral foregut, hepatoblasts and adherent passaged cells at different developmental stages were cultured for 2 days and then washed three times with methionine-free RPMI medium and labeled in RPMI medium supplemented with S-methionine (100 μCi/well) for 2 h.

The isolated mononuclear cells were resuspended in a serum-free medium and labelled with 10 μmol/L of BCECF-AM for 1 h at 37 °C.

Cells were suspended in complete medium and labelled with 90 μCi Chromium-51 (Amersham, Buckinghamshire, UK) at 37°C for 1 h with regular mixing.

AGS cells were suspended in the amount of 8 × 10 cells ml−1 in serum-free medium and labelled with 10  μ M BCECF/AM (Calbiochem, Darmstadt, Germany) by 30-min incubation at 37°C.

PNT2C2 cells were seeded (in triplicate) into 24-well plates at 2.5-, 5-, 7.5 × 10 and 1 × 10 in 0.5 ml R2.5 medium and labelled with 0.5  μCi [1-H]-Etn chloride (American Radiolabelled Chemicals, Stevenage, UK) per well for 36 h.

Cancer cells were harvested at full confluence, re-suspended at 1 × 10 cells ml−1 in medium and labelled with 2.5, 5, or 7.5  μg ml−1 Hoechst 33342 for 45, 60, or 90 min at 37°C to determine the required incubation time.

Light labeled cells were arrested in G0 phase while medium and heavy labeled cells were arrested in G2 and G1S phases, respectively.

Akt1 expressing HEK 293 cells were cultured in light, medium and heavy labeled SILAC media.