Sentence examples for medium and isolated from inspiring English sources
Cells were treated with 25 μM 3,5-DMAP for 1 h, washed with fresh medium, and isolated by centrifugation.
They were incubated overnight at 37°C in 10 ml of 2YT medium and isolated by standard alkaline lysis procedure [ 22].
The adaptive evolution experiments were performed with 40 passages in YNB medium and isolated clones were screened.
We cultured one set of four spores in a rich medium and isolated DNAs from these four cultures.
Supernatants from single cell suspensions of primary tumours as well as lymph node metastasis were collected 48 h after medium exchange and isolated T cells of healthy donors were incubated for 7 days with these supernatants (50% HNSCC supernatant+50% DMEM supplemented with IL-2) under TCR engagement.
One vast plain of industry and medium-rise housing and isolated shards of agriculture was succeeded by another.
Gene expression analysis using TaqMan Low Density Array (TLDA), single-cell suspension of spleens were prepared by gently teasing in RPMI medium and RNA isolated as above.
Strains were then passed once in medium without selection, subsequently streaked on solid medium containing 6% sucrose and isolated colonies were tested for loss of the gene by PCR (using primer pairs DelCheck1/DelCheck4 and DelCheck2/DelCheck3). Deletion of the gene was confirmed by sequencing.
3.9HG are semi-transformed cells generated by the transfection of NY317F cells with the TRK-T3 oncogene and isolated in medium supplemented with 5% calf serum plus hygromycin (25 μg ml−1) and G418 (400 μg ml−1).
Mauve colonies on Chromagar, or colonies suggestive of S. aureus on blood or Baird-Parker medium were isolated and confirmed with Vitek II (bioMérieux, Nürtingen, Germany) using GP and AST-P592 cards for species identification and antimicrobial susceptibility testing, respectively.
HeLa cDNA-expressing plasmids from clones growing on high stringency medium were isolated and retransformed into competent yeast expressing BD-IncA (aa 121-383).
