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A large fermentation vessel is filled with mineral medium and inoculated with a seed culture that contains bacteria.
The cells were re-suspended in the cultivation medium and inoculated such that an initial optical density of 0.05 was obtained.
Bone marrow was resuspended with cell culture medium and inoculated in gelatin-coated cell culture bottles at 37 °C and 5% carbon dioxide cell incubator.
After that, the cells were resuspended in the YNB medium and inoculated into the YNM medium to final density for cultivation (OD600 = 1.0).
Wells of a 96 well deep-well plate (2 ml well capacity, Whatman) were filled with 600 µl of medium and inoculated with the different yeast strains.
Subsequently, each bacterial suspension was diluted 10-fold in culture medium and inoculated in polystyrene 24-well-plates (Greiner Bio-One, Frickenhausen, Germany).
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Seeds were germinated on plant agar-medium and inoculated at three days post imbibition.
Each well was filled with 5 ml TSB-medium and inoculated with a pellet of R. microsporus mycelium from a 48 plate well.
The technique was tested on AAB grown in glucose medium (GY) and inoculated samples of red wine and wine vinegar.
Triplicate flasks containing 5 g corncobs were moistened (60% w/w) with Kirk's nutrient medium, sterilized and inoculated with 5 mL of freshly prepared homogeneous fungal spore suspension under sterile conditions in laminar air flow.
For liquid culture, spores were germinated in 2xYT medium [ 45] and inoculated into R5- liquid medium [ 20] lacking sucrose at a density of ~107 spores/ml.
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